Transfection Efficiency & Viability

Analyze post-transfection cytometric or manual counting data.

Total Cells Counted

Reporter+ Cells (e.g., GFP+)

Dead Cells (e.g., PI+)

Efficiency

0.0%

Viability

0.0%

Viable Transfected Yield

Balancing Toxicity and Efficacy

High transfection efficiency is often accompanied by high lipid/polymer toxicity. The true metric of a successful transfection protocol is the Viable Transfected Yield—the absolute number of cells that are both alive and expressing your construct.