Serial Dilution Workflow Planner

Plan multi-step dilution series for microplate assays and cell counting.

Stock Concentration

Dilution Factor (e.g., 10 for 1:10)

Final Volume per Tube (µL)

Number of Steps

Protocol: Transfer 100 µL into 900 µL of diluent for each step.

Dilution Theory

A serial dilution is a stepwise dilution of a substance in solution. Usually, the dilution factor ($DF$) is constant at each step. This geometry allows for the rapid generation of an expansive concentration range (logarithmic scale), essential for determining titer, IC50 values, or constructing standard curves.

Formulas used:
Transfer Volume = Final Volume / (DF - 1)
Diluent Volume = Final Volume
Concentration (Step n) = Stock / (DF)ⁿ

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