SDS-PAGE Gel Calculator
Determine optimal resolving power and generate custom gel casting recipes.
10%
Optimal Range: 20 - 150 kDa
Warning: Target sizes span a very wide mass range. Consider casting a Gradient Gel (e.g., 4-20%) for best resolution across all targets.
Resolving Gel Recipe
mL
| Component | Stock Concentration | Amount | |
|---|---|---|---|
| H₂O (deionized) | - | 4.00 | mL |
| Acrylamide/Bis | 30% (29:1) | 3.33 | mL |
| Tris-HCl (pH 8.8) | 1.5 M | 2.50 | mL |
| SDS | 10% | 0.10 | mL |
| APS (Fresh) | 10% | 0.10 | mL |
| TEMED | 100% | 10.0 | µL |
Note: Add APS and TEMED last, immediately before pouring the gel.