🧬 NGS Coverage Calculator
Plan and analyse your sequencing experiment: calculate depth, coverage, read requirements, and cost estimates.
💡 Instructions: Enter either the number of reads or desired coverage. The tool will compute the missing value and show all relevant metrics.
📖 Key Concepts
Coverage (depth): The average number of times each base is sequenced. For human whole-genome sequencing, 30X is typical. Exome sequencing often aims for 60-100X.
Total reads: The number of clusters passing filter. For a 30X human genome, you need ~900 million paired-end reads (150 bp).
Cost estimation: Based on typical sequencing pricing per Gb. This helps with grant proposals and budget planning.