Nearest-Neighbor Tm Calculator

Calculate precise DNA/RNA melting temperatures using thermodynamic models.

Primer Sequence (5' ➔ 3') Invalid characters detected. Use A, T, C, G only.

Primer Concentration (C) nM

Salt / Na⁺ Concentration mM

Length: 0 bp

GC Content: 0.0 %

Nearest-Neighbor T_m

0.0

°C (SantaLucia, 1998)

Enthalpy (ΔH)

0.0

kcal / mol

Basic T_m

0.0

°C (Wallace Rule)