🔬 Microscopy Image Analysis Simulator

Generate synthetic microscopy images and practice quantitative analysis – counting, intensity, segmentation, and more.

Hover over image to see pixel info

🧬 Cell Parameters

Number of cells 50 Cell size 20 Cell brightness 0.7 Background noise 0.1

⚙️ Thresholding (for analysis)

Threshold value 128

📊 Analysis Results

Cells

Mean Intensity

Total Area

Avg. Cell Area

% Coverage

💡 Instructions: Generate an image, adjust threshold, and click "Run Analysis" to quantify cell features.

📖 What you're learning

This simulator teaches the fundamentals of microscopy image analysis:
1. Segmentation – separating cells from background using thresholding.
2. Object counting – detecting and counting individual cells (nuclei).
3. Intensity measurement – quantifying fluorescence signal per cell.
4. Morphometry – measuring cell size, shape, and area.

These are the same principles used in professional software like ImageJ/FIJI, CellProfiler, and QuPath.