Flow Cytometry Compensation (2-Color)

Calculate true signals by mathematically eliminating spectral overlap.

Raw Signals (MFI)

Detector 1 Raw Signal Detector 2 Raw Signal

Spillover Matrix (%)

Fluor 1 spilling into Det 2 Fluor 2 spilling into Det 1

Compensated Fluor 1

Compensated Fluor 2

Matrix Math: True signals are found by multiplying the raw signal vector by the inverse of the spillover matrix. This isolates the actual fluorescence contributed by each specific dye.