Specific Enzyme Activity Calculator

Convert spectrophotometric rates into standard Volumetric and Specific Units (U/mg).

1. Spectrophotometer Data

Absorbance Change (ΔA) OD

Time Interval (Δt) Minutes

Extinction Coefficient (ε) M⁻¹ cm⁻¹

Path Length (l) cm

Total Assay Volume (V_t) µL

Enzyme Sample Added (V_s) µL

Protein Conc. in Stock mg / mL

Volumetric Activity

0.000

U / mL

Specific Activity

0.000

U / mg of Protein