🧬 Interactive Cloning & Primer Design

A step‑by‑step workflow for designing primers, simulating PCR, restriction digestion, and ligation.

1 Input Sequence
2 Enzymes & Vector
3 Results & Map

Step 1: Enter your DNA sequence

Paste the coding sequence (CDS) of your gene of interest below.

Step 2: Choose restriction enzymes & vector

Step 3: Results

πŸ§ͺ Primers Designed

🧬 PCR Simulation

βœ‚οΈ Restriction Digestion

πŸ”— Ligation & Plasmid Map